Recombinant Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] ab197234 adalah sejenis antibodi yang digunakan dalam eksperimen biologi untuk mendeteksi protein Ki67. Protein Ki67 adalah markah yang digunakan secara luas dalam bidang biologi sel dan histologi untuk menentukan tingkat proliferasi sel. Antibodi ini disebut "Anti-Ki67" karena mereka dirancang untuk mengenali dan berikatan dengan protein Ki67.
Baca Selengkapnya Minta PenawaranProduk data dan gambar Recombinant Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
14 Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234) Flow cytometry overlay histogram showing HeLa cells stained with ab197234 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab197234) (1x 106 in 100µl at 1µg/ml (1/500)) for 30min at 22°C. Isotype control antibody (black line) was Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab197234 staining Ki67 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197234 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
ab197234 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197234 at 1/100 dilution (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
Spesifikasi Recombinant Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
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Form
Liquid
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
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Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration
- 100 µl at 0.5 mg/ml
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