MACSQuant® Tyto® Cell Sorter
MACSQuant® Tyto® adalah revolutionizing cell sorting. Teknologi berbasis mikrocip yang dipatenkan kami membuka kemungkinan baru dalam penelitian dasar dan aplikasi medis. Mulailah memanfaatkan penyortiran aliran multiparameter berkecepatan tinggi dengan aman dalam kartrid yang sepenuhnya tertutup.
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Full sterility Samples are kept contamination-free within the disposable, fully closed MACSQuant Tyto Cartridge. No risk for carry-over or contamination of fluidics.
Fast and easy handling No drop delay or laser alignment needed. Simply insert the cartridge, gate on cells, and sort.
Gentle to cells Sort and even re.sort cells under low pressure without compromising cell viability or functionality. Cells are no longer exposed to high pressure, charge, or decompression.
Spesifikasi MACSQuant® Tyto® Cell Sorter
MACSQuant Tyto technical specifications
Optic
| Laser excitation | 405 nm, 100 mW |
| 488 nm, 100 mW | |
| 638 nm, 100 mW | |
| Emission detectors | V1: 450/50 nm |
| V2: 525/50 nm | |
| B1: 525/50 nm | |
| B2: 585/40 nm | |
| B3: 655–730 nm | |
| B4: 750 nm LP | |
| R1: 655–730 nm | |
| R2: 750 nm LP | |
| Backscatter and side scatter for detection of size & granularity of cells | |
| Fluorescence performance | 5 decade logarithmic scales, display in lin, log, or hlog scales |
Performance
| Purity | >99%* |
| Cell flow rate | Up to 55,000 cells/s for the MACSQuant Tyto Cartridge and up to 110,000 cells/s |
| for the MACSQuant Tyto Cartridge HS | |
| Sort rate | Up to 30,000 valve actuations/s |
| Viability | >99% for lymphocytes* |
| Sorting mode | Two-way sorting of sorted and non-sorted cells |
| Technical support | Built-in live support functionality for reliable support in real time |
| Operating pressure | <230 mbar (3 psi) with the MACSQuant Tyto Cartridge and 1100 mbar (<14 psi) |
| with the MACSQuant Tyto Cartridge HS | |
| Temperature control range | 4–25 °C (under standard environmental conditions) |
| Carry-over | No sample-to-sample carry-over due to single-use disposable cartridge |
Data management
| Measurement parameters | Height & Area measurements for all parameters |
| Signal processing | 16-bit analog-to-digital conversion and signal processing |
| Operating system | Core i7, 512 GB flash disk, 8 GB DDR4 RAM and Windows® 7 64-bit |
| Compensation | Full 8×8 matrix compensation |
| Data files | .mqd (proprietary file type); fcs (2.0, 3.0, 3.1 compatible) |
Operation details
| Dimensions | 965 × 585 × 280 mm3: without external monitor |
| 965 × 585 × 760 mm3: with external monitor | |
| Weight | 62 kg (137 lbs): without external monitor |
| 67 kg (148 lbs): with external monitor | |
| Monitors | 32 ̎ display and 15.6 ̎ touchscreen (internal) |
| Power requirements / consumption | 100-240 V, 50/60 Hz / 300 W: without external monitor |
| 100-240 V, 50/60 Hz / 500 W: with external monitor | |
| Ports | 4× USB 2.0, 6× USB 3.0, 2× Ethernet, DVI-D |
Environmental conditions
| Operational temperature / humidity | 18–25 °C / 0–85% relative humidity, non-condensating |
| Emission sound pressure < 70 dB(A) level at workstation | <70 dB(A) |
Cartridge details
| Sterility | Ethylene oxide-validated sterilization method to guarantee sterility of each cartridge |
| A-septic filters | 0.1 µm hydrophobic filters at air inlet and outlet ports |
| Sorting channel width | 25 × 50 µm |
| Maximum loading volume (input) | 100 µL–10 mL |
| Maximum loading cell number | 5×107 cells/mL |
| Cartridge alignment for laser positioning | Continuous automated cartridge alignment also during the sort |
| Cell retrieval | No cell loss - negatively sorted cells are kept in the negative collection chamber |
| Barcode labeling | Cartridges are labeled with a barcode for easy identification and traceability |